Наукова періодика України | Experimental oncology | ||
Kochubei T. O. Isolectins of phytohemagglutinin are able to induce apoptosis in HEP-2 carcinoma cells in vitro / T. O. Kochubei, O. V. Maksymchuk, O. O. Piven, L. L. Lukash // Experimental oncology. - 2015. - Vol. 37, № 2. - С. 116-119. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2015_37_2_8 Aim - to study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. The commercial preparation of the kidney beans (Phaseolus vulgaris) lectins and HEp-2 cells were used. Apoptosis index was determined using acridine orange and ethidium bromide staining. The expression levels of apoptosis mediator cleaved caspase-3 and proapoptotic Bax protein were studied by Western blot analysis. The gene expression levels were analyzed by qPCR. PHA and its isolectins induced apoptosis in HEp-2 cells accompanied by the increased expression of caspase-3 cleaved form, with PHA-E being the most effective. The treatment of HEp-2 cells with PHA or its isolectins resulted in a marked increase of Bax on both mRNA and protein levels. Conclusions: PHA and its isolectins were shown to induce the apoptosis in human HEp-2 carcinoma cells via increasing proapoptotic protein Bax and activating caspases-3. Цитованість авторів публікації: Бібліографічний опис для цитування: Kochubei T. O. Isolectins of phytohemagglutinin are able to induce apoptosis in HEP-2 carcinoma cells in vitro / T. O. Kochubei, O. V. Maksymchuk, O. O. Piven, L. L. Lukash // Experimental oncology. - 2015. - Vol. 37, № 2. - С. 116-119. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2015_37_2_8. Якщо, ви не знайшли інформацію про автора(ів) публікації, маєте бажання виправити або відобразити більш докладну інформацію про науковців України запрошуємо заповнити "Анкету науковця"
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