The diversity of cultured pathogenic bacteria in the bottled mineral water (BMW) was investigated using selective media. The pure isolates from these selective media, which showed hemolytic activity on the blood agar media and antibiotic resistance, were identified by 16S rRNA gene technique. The seven obtained strains were belonged to the genus Pseudomonas, Bacillus, Acinetobacter, Stenotrophomonas, and Exiguobacterium, and were mostly closed to the pathogenic strains. The increasing of ozone concentration from air-fed ozone generators eliminate the growth of bacteria included the pathogenic bacteria, but in other side it increases the amount of nitrates and nitrites in the final product of the BMW. These findings revealed that the BMW either has potential pathogenic bacteria or high levels of nitrates and all these products may effect on the health of the end user.