Zhuravel O. V. Expression of human beta-defensins-1-4 in thyroid cancer cells and new insight on biologic activity of hBD-2 in vitro / O. V. Zhuravel, O. L. Gerashchenko, M. R. Khetsuriani, M. A. Soldatkina, P. V. Pogrebnoy // Эксперим. онкология. - 2014. - 36, № 3. - С. 174-178. - Бібліогр.: 22 назв. - англ.The study was aimed on analysis of human beta-defensin-1-4 (hBDs) mRNA expression in cultured thyroid cancer cells and evaluation of effects of recombinant hBD-2 (rec-hBD-2) on growth patterns, migration properties and expression of E-cadherin and vimentin in these cells. The study was performed on cultured follicular thyroid cancer WRO cells, papillary thyroid cancer TPC1 cells, and anaplastic thyroid cancer KTC-2 cells. For analysis of hBD-1-4 mRNA expression in thyroid cancer cells, scmiquantitative RT-PCR was used. Effects of rec-hBD-2 on cell proliferation, viability, and migration were analyzed using direct cell counting, MTT test, and scratch assay respectively. Expression of vimentin and E-cadherin was evaluated by quantitative PCR (qPCR). By the data of RT-PCR, ah three studied thyroid cancer cell lines express hBD-1 and -4 mRNA, but not hBD-2 mRNA, whOe hBD-3 expression was detected in WRO and KTC-2 cells. The treatment of TPC-1, WRO, and KTC-2 cells with 100 - 1000 nM rec-hBD-2 resulted in significant concentration-dependent suppression of ceU proliferation, viability, and migratory property. By the data of qPCR, significant up-regulatkm of vimentin expression was registered in KTC-2 and WRO cells treated with 500 nM rec-hBD-2. Significant down-regulation of E-cadherin expression (p << 0,05) was detected only in KTC-2 cells treated with the defensin. Also, it has been shown, that TPC-1 cells treated with 500 nM rec-hBD-2 acquired more elongated morphology. Conclusion: the data demonstrate that hBD-2 in concentrations higher than 100 nM exerts significant concentration-dependent suppression of thyroid cancer cell growth and migration, and affects vimcntin and E-cadherin expression dependent on histologic type of thyroid cancer cells. Індекс рубрикатора НБУВ: Р569.452
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