The expression of multiple molecular forms of adaptor proteins Ruk/CIN85 and CD2AP/CMS was studied in Triton X-100-soluble extracts and extracts of total tissue protein using Western-blot analysis. Differently directed changes in the expression level of Ruk/CIN85 full-length form and CD2AP/CMS were revealed in samples of multinodular goitre, follicular adenoma and papillary carcinoma in comparison with normal tissue (morphologically non-changed tissue isolated from the same operated organ). According to the data obtained, full-length form of Ruk/CIN85 is localized mainly in the cytoplasm of control samples whereas in pathologically changed tissues this form is strongly associated with elements of cell cytoskeleton and/or nuclear matrix. Meanwhile, the form without first SH3A domain (p70) is strongly associated with cell components insoluble in Triton X-100 both in normal and transformed tissues. Increase in the expression level of full-length Ruk/CIN85 form was found in the central area of thyroid papillary carcinoma samples in comparison with peripheral area and control tissue.

  Якщо, ви не знайшли інформацію про автора(ів) публікації, маєте бажання виправити або відобразити більш докладну інформацію про науковців України запрошуємо заповнити "Анкету науковця"