Introduction. There is a serious worldwide protein deficiency problem, so it is the obligation to know about edible species and their biochemical composition. Sea cucumbers have great value because of its high protein content. The study comprises monthly data on biochemical content of H. tubulosa. Materials and methods. Monthly variations in biochemical composition of sea cucumbers and environmental interrelationship were investigated from April 2013 to March 2014, from three stations (Gelibolu, Umurbey and Dardanos) in Dardanelles Strait, Turkey. Results and discussion. Temperature pattern was similar at the stations while salinity and organic matter values at Dardanos was different than others stations. Protein was maximum (52,48 %) in March at Gelibolu; in May (52,81 %) at Umurbey and (56,93 %) at Dardanos; lipid was maximum (1,70 %) in February at Gelibolu; in March (1,77 %) at Umurbey and in September (1,42 %) at Dardanos; ash was maximum (43,51 %) in November at Gelibolu; in September (44,22 %) at Umurbey and in December (44,98 %) at Dardanos. Carbohydrate reserves were reduced due to being used as energy for lipid synthesis in September and also carbohydrate and lipid reserves could be used as an energy source during periods of lower available nutrition for basic metabolic function. Protein, lipid, carbohydrate, ash and moisture values varied depending on food supply and reproduction period and might have been indirectly affected by environmental conditions. Conclusions. The study clearly showed that protein, lipid, carbohydrate and ash values varied depending on food supply and reproduction period and might have been indirectly affected by environmental conditions.

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