Purpose. To perform a quantitative and qualitative impact assessment from point and diffuse sources of anthropogenic pollution on the Prut River basin within Ukraine. Methodology. Based on the developed statistical information and the use of the Guidance Paper on Impact Analysis and Risk Assessment in accordance with the EU Water Directive (EU project "Environmental protection of international river basins-), load calculations have been carried out on four factors influencing the Prut ecosystem, namely, volumes of discharges of untreated sewage, the total share of sewage in the river, from agriculture and livestock production. Findings. The main anthropogenic loads on the Prut river basin within Ivano-Frankivsk and Chernivtsi regions of Ukraine from point and diffuse sources have been analyzed and calculated. In fact, it has been confirmed that diffuse sources are the main pollutants of the Prut ecosystem. Non-canalized area caused the emission of organic and nutrient compounds into the river basin. According to the calculation of the indicators of diffuse load, water massifs in Kitsman, Kolomyia and Snyatyn districts are "possibly at risk"from agriculture, and in Hertzayivsky district - from livestock. The largest point polluters in the Prut River are communal enterprises of the settlements in Kolomyia and Chernivtsi. Originality. The methodology for estimating loads on the river basin has been further developed by calculating load indicators, which allows the development and implementation of effective methods and tools for controlling the ecological safety of aquatic ecosystems. Practical value. The results obtained are the basis for adapting European approaches to integrated water resources management and developing measures to minimize the risks from human activities in the Prut basin. The work has been implemented at the national level in the process of drafting the Prut River Basin Management Plan in the framework of the international project "Environmental protection of international river basins"(EPIRB) and improvement of the water monitoring system in the Carpathian National Nature Park.

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