Aim - to analyze biological characteristics, malignant potential and expression of proteins associated with epithelial-mesenchymal transition in murine lung carcinoma cells transduced with interferon-beta (ifn-beta) gene in baculovirus vector. The study was performed on Lewis lung carcinoma (LL) cells transduced with ifn-beta gene in recombinant baculovirus vector. Biological characteristics of the LL cells were studied with the use of standard cell culture methods, cytogenetic and immunocyto-chemical assays. Recombinant baculovirus-mediated transduction of LL cells with ifn-beta gene resulted in significant decrease of cell growth rate and density both in complete and serum-free medium. Also, LL cells transduction with ifn-beta gene significantly inhibited cell migration in vitro. Transduction of LL cells by baculovirus vector with or without ifn-beta gene caused significant genotoxic effect in these cells. Furthermore, ifn-beta gene transfer to lung carcinoma cells resulted in significant increase of nuclear expression ofp19<^>ARF (p << 0,01), p21<^>WAF1 (p << 0,001), cytoplasmic expression of E-cadherin (p << 0,005) and inhibition of transcription factors of epithelial-mesenchymal transition (EMT) Twist (p << 0,005) and Slug (p << 0,001) expression. Conclusions: transduction with ifn-beta gene of LL cells in recombinant baculovirus resulted in acquirement of less malignant phenotype in vitro and suppressed expression of proteins associated with EMT.

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