Green tea, red wine and lemon extracts reduce experimental tumor growth and cancer drug toxicity

Zaletok, S.; Gulua, L.; Wicker, L.; Shlyakhovenko, V.; Gogol, S.; Orlovsky, O.; Karnaushenko, O.; Verbinenko, A.; Milinevska, V.; Samoylenko, O.; Todor, I.; Turmanidze, T.

Aim - to evaluate antitumor effect of plant polyphenol extracts from green tea, red wine lees and/or lemon peel alone and in combination with antitumor drugs on the growth of different transplanted tumors in experimental animals. Green tea extract (GTE) was prepared from green tea infusion. GTE-based composites of red wine (GTRW), lemon peel (GTRWL) and/or NanoGTE as well as corresponding nanocomposites were prepared. The total polyphenolics of the different GTE-based extracts ranged from 18,0 % to 21,3 %. The effects of GTE-based extracts were studied in sarcoma 180, Ehrlich carcinoma, B16 melanoma, Ca755 mammary carcinoma, P388 leukemia, L1210 leukemia, and Guerin carcinoma (original, cisplatin-resistant and doxorubicin-resistant variants). The extracts were administered as 0,1 % solution in drinking water (0,6 - 1,0 mg by total polyphenolics per mouse per day and 4,0 - 6,3 mg per rat per day). Tumor growth inhibition (TGI) in mice treated with NanoGTE, cisplatin or cisplatin + NanoGTE was 27 %, 55 % and 78 %, respectively, in Sarcoma 180 %, 21 %, 45 % and 59 %, respectively, in Ehrlich carcinoma; and 8 %, 13 % and 38 %, respectively in B16 melanoma. Composites of NanoGTE, red wine, and lemon peel (NanoGTRWL) enhanced the antitumor effects of cyclophosphamide in mice with Ca755 mammary carcinoma. The treatment with combination of NanoGTE were tested against cisplatin- or doxorubicin-resistant Guerin carcinoma. Moreover, the plant extracts lowered side toxicity of the drugs. Treatment with GTE, NanoGTE, and NanoGTRW decreased the levels of malondialdehyde in heart, kidney and liver tissue of experimental animals, as well as the levels of urea and creatinine in blood serum, increased erythrocyte and platelet counts, hemoglobin content, and decreased leucocyte counts. Conclusion: the obtained data indicate the prospects for further development of GTE and corresponding nanocomposites as auxiliary agents in cancer chemotherapy.


	Наукова періодика України		Experimental oncology