Aim - the aim of the study was to determine the phosphorylated c-Jun content and reduced and oxidized glutathione (GSH/GSSG) ratio in gastric mucosa cells during the process of gastric cancer development in rats. Gastric carcinogenesis was initiated in 80 white male rats by 10-week replacement of drinking water with 0,01 % solution of N-methyl-N'-nitro-N-nitrosoguanidine, at die same time they were redefined on diet containing 5 % NaCl. Then the animals were fed with standard vivarium diet till the end of 24^th week. The gastric mucosa cells were examined at the end of 4^th, 6^th, 8^th, 10^th, 12^th, 18^th, and 24^th weeks. Sandwich ELISA method was used to determine the content of phospho-c-Jun. The contents of GSH and GSSG were analyzed by spectrofluorymetric method with the use of orthophthalic aldehyde. At the end of 6^th, 8^th, 10^th weeks of MNNG and NaCl treatment the gastric mucosa cells were characterized by 4-, 6,3-, 1,9-fold higher content of phospho-c-Jun compared to the control, respectively, and 12, 18 and 24 weeks there was registered a stable increase of phospho-c-Jun content on the average at 3,6-fold compared to control values. Starting from 6^th week of gastric cancer development an average decrease of GSH/GSSG was 3,4-fold compared with the control. Conclusion: during gastric carcinogenesis there was registered the decrease of GSH/GSSG ratio and increased level of phosphorylated c-Jun what points on MAP-kinase cascade activation in prooxidant conditions.

  Повний текст PDF - 109.475 Kb    Зміст випуску     Цитування публікації

  Якщо, ви не знайшли інформацію про автора(ів) публікації, маєте бажання виправити або відобразити більш докладну інформацію про науковців України запрошуємо заповнити "Анкету науковця"